Cytotoxicity of Five Endodontic Sealers in NIH 3T3 Fibroblasts

The objective of this study is to assess the cytotoxic effects of substances leached from five endodontic sealers in time periods of 0 and 24 hours on NIH 3T3 fibroblasts. It was used a medium conditioned by the sealers, subdivided in six groups namely: Control (fresh cell culture medium); SimpliFill (Discus Dental, Culver City, Calif) : EndoREZ ® (Utradent Products, Inc); EPIPHANY® (Pentron Clinical Technologies, Wallingford, CT, USA); EPIPHANY® SE (Pentron Clinical Technology); AH Plus® ((Dentsply/Caulk, Petrópolis, RJ, Brazil) in intervals of 0 (freshly made) and 24 hours (set form). The obtained data were interpreted by the one-way ANOVA and Tukey test, respectively; with a level of significance of 5% (p≤0.05). The mean number of viable cells was 0.3208 for the control group, followed by freshly made SimplyFill (0.1818) and its set form (0.1800); freshly made EndoREZ (0.0899) and its set form (00.1465); freshly made Epiphany (0.2138) and its set form (0.1795); freshly made Epiphany SE (0.1505) and its set form (0.1463); and freshly made AH-Plus (0.2480) and its set form (0.2265). Statistical differences were found among the groups (p≤0.05), with the cell viability decreasing significantly with EndoREZ in its fresh form (p p≤0.01) and in its set form (p<0.05); followed by EndoREZ in both fresh and set forms (p p≤0.05), when compared to SimplyFill, Epiphany, AHplus and the control groups. EndoREZ was the most cytotoxic sealer of all tested. The observed differences among the cytotoxicity concluded that EndoREZ was the most cytotoxic sealer of the five tested in the experiment in time the time intervals of 0 and 24 hours.